| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-CherryPicker Control |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8998 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | CherryPicker (C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-CherryPicker Control是pLVX-CherryPicker1和pLVX-CherryPicker2的阳性对照载体。 |
| 产品目录号: | 632580 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-CherryPicker_Control 8999 bp DNA circular 11-APR-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 632580,632574,632581,632575
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 4967..5558
/label=WPRE
rep_origin complement(6867..7540)
/label=pUC\origin
CDS complement(7685..8681)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5761..6397
/label=3'\LTR
misc_feature 3825..4333
/label=PGK\Promoter
misc_feature 4354..4953
/label=Puro(R)
CDS 2885..3811
/label=CherryPicker
misc_feature 3812..3814
/label=Stop\codon
BASE COUNT 2264 a 2310 c 2406 g 2019 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcg gccgcgttta
2881 aacgatgatg gtggacggcg acaacagcca cgtggagatg aagctggccg tggacgagga
2941 agagaacgcc gacaacaaca ccaaggccaa cgtgaccaag cccaagcggt gcagcggcag
3001 catctgctac ggcacaatcg ccgtgatcgt gttcttcctg atcggcttca tgatcggcta
3061 cctgggctac tgcaagtcta gcgacggccc tggcgagaca ggcatggtgt ccaagggcga
3121 agaggacaac atggccatca tcaaagaatt catgcggttc aaggtgcaca tggaaggcag
3181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgaa ggcagacctt acgagggcac
3241 ccagaccgcc aagctgaaag tgaccaaggg cggacccctg ccctttgcct gggacatcct
3301 gagcccccag ttcatgtacg gcagcaaggc ctacgtgaag caccccgccg acatccccga
3361 ctacctgaag ctgagcttcc ccgagggctt caagtgggag agagtgatga acttcgagga
3421 cggcggcgtg gtcaccgtga cccaggacag cagcctgcag gacggcgagt tcatctacaa
3481 agtgaagctg cggggcacca acttccctag cgacggcccc gtgatgcaga aaaagaccat
3541 gggctgggag gccagcagcg agagaatgta ccccgaggat ggcgccctga agggcgagat
3601 caagcagcgg ctgaagctga aggatggcgg ccactacgac gccgaagtga aaaccaccta
3661 caaggccaag aaacccgtgc agctgcctgg cgcctacaac gtgaacatca agctggacat
3721 caccagccac aacgaggact acaccatcgt ggagcagtac gagagagccg agggcagaca
3781 cagcaccggc ggcatggacg agctgtacaa atgacaatct agataattct accgggtagg
3841 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
3901 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
3961 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
4021 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
4081 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
4141 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
4201 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
4261 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
4321 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
4381 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
4441 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
4501 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
4561 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
4621 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
4681 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
4741 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
4801 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
4861 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
4921 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga
4981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
5041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
5101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
5161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
5221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
5281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
5341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
5401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
5461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
5521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
5581 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
5641 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
5701 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
5761 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
5821 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
5881 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
5941 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
6001 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
6061 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
6121 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
6181 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
6241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
6301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
6361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
6421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
6481 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
8701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
8821 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
8881 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
8941 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
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